streptavidin biotin unlabeled immunoperoxidase technique (Vector Laboratories)

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Vector Laboratories streptavidin biotin unlabeled immunoperoxidase technique
Streptavidin Biotin Unlabeled Immunoperoxidase Technique, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 2621 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/streptavidin+biotin+peroxidase+kit/VECTASTAIN+Elite+ABC+HRP+Kit+(Peroxidase%2C+Rabbit+IgG)/pm41780809-133-1-8
Average 96 stars, based on 2621 article reviews

streptavidin biotin unlabeled immunoperoxidase technique - by Bioz Stars, 2026-09

96/100 stars

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Binding Assay:

Article Title: Pathological Features and Molecular Phenotype of MMTV Like-Positive Feline Mammary Carcinomas
Article Snippet: .. Antibody binding was detected using a streptavidin-biotin-peroxidase kit (Vector Laboratories, Burlingame, CA, USA). .. Further, 3,30-diamino-benzidine (0.05% for 10 min at RT) was used as chromogen (Vector Laboratories, CA, USA).

Article Title: Experimental infection with Yersinia pseudotuberculosis in European brown hare (Lepus europaeus, Pallas).
Article Snippet: .. A commercial streptavidin-biotin-peroxidase kit (Vector Laboratories, Inc., Burlingame, CA, USA) was used to detect specific antibody binding. previously applying a 1:200 dilution of A biotinylated goat antimouse immunoglobulin was applied as a secondary antibody (AO433; DakoCytomation, Denmark) with a dilution of 1:200 for 45 min at room temperature. .. The enzymatic reaction was developed with the use of 3-1-diaminobenzydine (DAB; SigmaChemical Co., St. Louis,Missouri, USA).

Article Title: Glue and Ethanol Mixture for Aneurysm Endovascular Treatment: Animal Lab Study, Imaging, and Histopathological Findings
Article Snippet: Anti-Iba1 rabbit polyclonal antobody (Wako, Neuss, Germany; dilution 1:300), anti-CD3 rabbit polyclonal antibody (Dako, Glostrup, Denmark; dilution 1:200), and anti-Cd79a mouse monoclonal antibody (Exbio, Praha, Czech Republic; dilution 1:100) were used for macrophages, T-lymphocytes, and B-lymphocytes, respectively. .. Antibody binding was detected by the Biotinylated horse anti-Mouse/Rabbit IgG antibody (H + L) R.T.U. (Vectors Laboratories, CA, USA), the streptavidin-biotin-peroxidase kit (Vector Laboratories, Newark, CA, USA) and the 3,30-diamino-benzidine as chromogen (Vector Laboratories, Newark, CA, USA) as indicated by the manufacturer’s instructions. ..

Article Title: Glue and Ethanol Mixture for Aneurysm Endovascular Treatment: Animal Lab Study, Imaging, and Histopathological Findings.
Article Snippet: Anti-Iba1 rabbit polyclonal antobody (Wako, Neuss, Germany; dilution 1:300), anti-CD3 rabbit polyclonal antibody (Dako, Glostrup, Denmark; dilution 1:200), and antiCd79a mouse monoclonal antibody (Exbio, Praha, Czech Republic; dilution 1:100) were used for macrophages, T-lymphocytes, and B-lymphocytes, respectively. .. Antibody binding was detected by the Biotinylated horse anti-Mouse/Rabbit IgG antibody (H + L) R.T.U. (Vectors Laboratories, CA, USA), the streptavidin-biotin-peroxidase kit (Vector Laboratories, Newark, CA, USA) and the 3,30-diamino-benzidine as chromogen (Vector Laboratories, Newark, CA, USA) as indicated by the manufacturer’s instructions. ..

Incubation:

Article Title: ALKBH4 promotes tumourigenesis with a poor prognosis in non-small-cell lung cancer
Article Snippet: .. The sections were blocked with 1% bovine serum albumin and were incubated with the rabbit polyclonal antibody against human ALKBH4 (1:200; Novus Biologicals, NBP2-14737) overnight at 4 °C, followed by staining with a streptavidin–biotin peroxidase kit (Vector Laboratories, CA, USA). ..

Article Title: Clinical significance of ALKBH4 expression in non-small cell lung cancer
Article Snippet: .. The blocked sections were incubated with the rabbit polyclonal antibody against human ALKBH4 (1:200; Novus Biologicals, Littleton, CO, USA, NBP2-14737) and left overnight at 4 °C, followed by staining with a streptavidin-biotin peroxidase kit (Vector Laboratories, Inc., Burlingame, CA, USA). ..

Staining:

Article Title: IκB kinases increase Myc protein stability and enhance progression of breast cancer cells
Article Snippet: .. IHC staining was performed using a streptavidin-biotin-peroxidase kit(Vectastain Universal Quick Kit; Vector Laboratories, Burlingame, CA, USA) according to the manufacturer's instructions. .. DAB/chromogen system (Dako Northern America, Inc.)was used to develop the image.

Immunohistochemistry:

Expressing:

Article Title: Afobazole alleviates streptozotocin-induced diabetic nephropathy in rats via hypoglycemic, antioxidant, anti-inflammatory, and anti-apoptotic properties: Role of the S1R/Nrf2 antioxidant axis.
Article Snippet: Aims: Sigma-1 receptor (S1R) activation was recently identified as a promising target for preventing diabetic nephropathy (DN) by mitigating hypoxia, oxidative stress, and inflammation.. This study aimed to investigate the potential reno-protective effect of the S1R agonist afobazole against streptozotocin (STZ)-induced DN in rats compared to metformin.. Materials and methods: Rats were split into six groups: the normal control group; the diabetic control group received STZ (55 mg/kg i.p.); the other four groups received STZ and were treated with different doses of either afobazole (10, 15, and 20 mg/kg) or metformin (200 mg/kg).

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